Apr 26, 2019 · With the use of certain solvents, may also be used to stain some protein bound lipids in paraffin sections. Sudan I (sc-215922), Sudan III (sc-203761), and Sudan IV (sc-203762) have been classified as category 3 carcinogens by the International Agency for Research on Cancer. References. 1. Kodama, H., et al. 1977. J. Dermatol. 4: 41-48. PMID ... The tests performed were, iodine testing for starch and glycogen, Benedict’s test for reducing sugars, and the Biuret’s test for protein. Depending on the test performed, certain results acquired, clearly identified the macromolecules present in each solution. With the aid of the results from the lab, the unknown solution will also be determined. Lipid Test Station: Fat - Sudan III stain. Sudan III is used to identify the presence of lipids in liquids. It will stain fat cells red. What to do. Place three test tubes in a test tube rack and label them control (distilled water), lipid (distilled water + oil), unknown (stomach contents). To the first test tube, add 2 ml of distilled water.